hantavirus pcr lab test

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  • Feb 03, 2020

Laboratory methods used for hantavirus diagnostics in clinical ...
Laboratory methods used for hantavirus diagnostics in clinical …

The results of serologic tests positive, evidence of viral antigen in tissues by immunohistochemistry, or the presence of viral RNA sequences amplifiable in the blood or tissues, with a compatible history of HPS, is considered diagnostic for HPS.

during the outbreak in the Four Corners area, cross-reactive antibodies to hantaviruses previously known (for example, Hantaan, Seoul, Puumala and Prospect Hill virus) found in acute- and healing-phase sera of some 1993 patients HPS early. Tests based on viral antigen from SNV has since been developed and are now widely used for routine diagnosis of HPS. CDC using enzyme-linked immunosorbent assay (ELISA) for the detection of IgM antibodies to SNV and for diagnosing acute infection with other hantaviruses. This test is also available in some countries health laboratory. test

A used along with test IgG IgM-capture. Acute- and cured-phase sera should reflect the four-fold rise in antibody titer IgG or IgM presence in the acute phase sera be diagnostic for disease hantaviral. Note that the acute phase serum specimens sent as an early diagnostic may not have IgG. IgG antibodies durable, and sera of patients were retrospectively identified antibody seems to have retained over the years. The SNV IgG ELISA has therefore been used in the investigation of serological epidemiology of the disease and it seems appropriate for this purpose. Investigation of selected population using this test has confirmed that infection with a virus that is not common and that mild or asymptomatic infections are rare.

A Western blot assay using recombinant antigen and conjugate isotype-specific IgM-IgG for differentiation has also been developed and the results are generally in agreement with those of the IgM-capture format.

Also used is a rapid test strip immunoblot (RIBA), a test prototype examined to identify serum antibodies to recombinant proteins and peptides specific for SNV and other hantaviruses.

serological confirmation hantaviral infection has traditionally been carried out by neutralizing the test plaque, which has been recently described for SNV. However, this particular test is also not commercially available.

Insulation hantaviruses (see below) from human sources is difficult, and the virus that causes HPS seems to be no exception to this rule. To date, no isolates SNV-like virus has been recovered from humans, and therefore virus isolation is not a consideration for diagnostic purposes.

IHC testing formalin-fixed tissue with specific monoclonal and polyclonal antibodies can be used to detect hantavirus antigens and has proven to be a sensitive method for laboratory confirmation of infection hantaviral. IHC has an important role in the diagnosis of HPS in patients from whom serum samples and frozen tissue is not available for diagnostic testing and in a retrospective assessment of the prevalence of the disease in the defined geographical area.

reverse transcriptase-PCR (RT-PCR) can be used to detect RNA in fresh tissue frozen hantaviral the lungs, blood clots, or nucleated blood cells. However, RT-PCR is very susceptible to cross-contamination and should be considered an experimental technique. Differences virus in the United States complicates the use and sensitivity of RT-PCR for routine diagnosis hantaviral infection.

high-power magnification shows immunostaining of renal interstitial capillary using serum Peromyscus (Click the image for a larger view) image belongs to Sherif R. Zaki, MD, Ph.D.

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